Nine days later, the company announced that it will close the location within six months.
A spokesman for the taking a watch centre suggested the BBC that "the fine thing to attain is to handiest allow Chick-fil-A to trade with us for the preliminary six-month pilot length, and no longer to expand the lease any further".
LGBTQ organization Reading Pride - which spearheaded the protests - said the mall's decision was "good news", as it allowed time for employees to find other work.
According to Berkshire Live, Reading diners were "deeply divided" and engaged in a "fierce debate" about the opening.
In 2012, now-CEO Dan Cathy said the company believed in the "biblical definition of the family unit".
The 519, an organization dedicated to advocacy for the inclusion of LGBTQ2 communities, along with LiberationTO and animal rights activists, made up the protest group and stood outside with signs, photos and chants.
Chick-fil-A has previously come forward to defend themselves to BBC, saying "There are 145,000 people-black, white; gay, straight; Christian, non-Christian-who represent Chick-fil-A".
In 2012, the company's chairman sparked a U.S. boycott when he said he opposed gay marriage. We regard everyones opportunity to eat where they pick, in any case, we ask the LGBT+ people group (counting partners) to blacklist the chain in Reading.
Chick-fil-A declined to respond on the record to TIME's question as to whether the company had been planning to extend the lease after the six month pilot period. Five citizens demanded that an injunction should be brought against the airport barring any company "based wholly or partly on that person or entity's support for religious organisations that oppose homosexual behaviour".
Samuel Cathy Snr is also reported to have used Chick-fil-A profits to fund charities such as Exodus International, a group which promotes gay conversion therapy.
Chick-fil-A responded to Reading Pride's campaign by refuting claims that it had anything to do with "legislative campaigns" in Uganda.